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Processed directly from adult human skin (from healthy donors) and expanded in vitro for a maximum of three passages in the presence of a lethally irradiated 3T3 feeder cell layer, by following the classical procedure of Rheinwald and Green (1975).
Human keratinocytes are characterised morphologically through serial passage, and each batch is tested for expresion of the following keratinocyte markers: cytokeratin 14 (K14), integrin alpha-6 (CD49f), integrin beta-1 (CD29).
All cells have been processed in INBIOBANK by manufacturing procedures based on ISO9001:2000 standards. These cells have tested negative for the following pathogens: HIV-1/2, Hepatitis B/C (Abbot Prism Chemiluminiscence Assay), and Mycoplasma (PCR Kit).
 
 
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